Plasmid
Part:BBa_K4047036:Design
Designed by: An Nguyen Group: iGEM21_MiamiU_OH (2021-10-06)
pGEM-tal
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 7968
Illegal XbaI site found at 2
Illegal XbaI site found at 2546
Illegal XbaI site found at 2860
Illegal PstI site found at 14
Illegal PstI site found at 2558
Illegal PstI site found at 5013
Illegal PstI site found at 5815 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 7968
Illegal NheI site found at 2673
Illegal NheI site found at 8658
Illegal PstI site found at 14
Illegal PstI site found at 2558
Illegal PstI site found at 5013
Illegal PstI site found at 5815
Illegal NotI site found at 515 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 7968
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 7968
Illegal XbaI site found at 2
Illegal XbaI site found at 2546
Illegal XbaI site found at 2860
Illegal PstI site found at 14
Illegal PstI site found at 2558
Illegal PstI site found at 5013
Illegal PstI site found at 5815 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 7968
Illegal XbaI site found at 2
Illegal XbaI site found at 2546
Illegal XbaI site found at 2860
Illegal PstI site found at 14
Illegal PstI site found at 2558
Illegal PstI site found at 5013
Illegal PstI site found at 5815
Illegal NgoMIV site found at 1043
Illegal NgoMIV site found at 3454
Illegal NgoMIV site found at 8493 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 198
Illegal BsaI site found at 2068
Illegal BsaI site found at 9117
Illegal BsaI.rc site found at 4839
Illegal BsaI.rc site found at 5605
Illegal SapI site found at 3756
Design Notes
Source
Gene sourced from Synechococcus elongatus PCC 7942 Genbank genome sequence for transaldolase, at the locus tag Synpcc7942_2297. https://www.ncbi.nlm.nih.gov/nuccore/CP000100.1?from=2367528&to=2368721&report=fasta&strand=2
Backbone sourced from pAM2991 created by Susan Golden in 2005 for work in S. elongatus PCC 7942.
https://www.addgene.org/40248/